《植物生理学报》 2014, 50(1): 1-6

园艺植物茎尖冷冻疗法脱毒的技术研究

邱静, 汤浩茹, 曹会娟, 许晶, 杨海燕, 罗娅*

四川农业大学园艺学院, 四川雅安625014

通信作者：罗娅；E-mail: 173698873@qq.com；Tel: 13111822452

摘　要：

超低温冷冻疗法是超低温保存技术在植物脱毒上的一个新的应用。由于其脱毒率高, 操作简单, 被认为是最有效脱除植物病毒的方法, 为脱毒苗的生产开辟了一条新的途径。本文就超低温冷冻疗法脱毒的原理、操作程序、关键技术和在园艺植物上的最新研究进展进行综述, 并对其今后的发展进行了展望。

关键词：超低温疗法; 超低温保存; 茎尖; 病毒

收稿：2013-10-23   修定：2013-12-04

资助：四川农业大学“双支计划”项目(03570109)和四川农业大学大学生创新性实验计划(6309215)

Study on Cryotherapy of Shoot Tips of Horticultural Plants

QIU Jing, TANG Hao-Ru, CAO Hui-Juan, XU Jing, YANG Hai-Yan, LUO Ya*

College of Horticulture, Sichuan Agricultural University, Ya’an, Sichuan 625014, China

Corresponding author: LUO Ya; E-mail: 173698873@qq.com; Tel: 13111822452

Abstract:

Cryotherapy is a novel application of plant cryopreservation in virus elimination. Due to high virusfree percentage and easy operation, it is considered to be the most effective detoxification method, and create a new path for the production of virus-free plantlets. In this paper, the principles, operating procedures, key technology and the latest research progress in horticultural plants of cryotherapy were summarized; the future development of cryotherapy was discussed as well.

Key words: cryotherapy; cryopreservation; shoot tips; virus