《植物生理学报》 2014, 50(1): 1-6
通信作者:罗娅;E-mail: 173698873@qq.com;Tel: 13111822452
摘 要:
超低温冷冻疗法是超低温保存技术在植物脱毒上的一个新的应用。由于其脱毒率高, 操作简单, 被认为是最有效脱除植物病毒的方法, 为脱毒苗的生产开辟了一条新的途径。本文就超低温冷冻疗法脱毒的原理、操作程序、关键技术和在园艺植物上的最新研究进展进行综述, 并对其今后的发展进行了展望。关键词:超低温疗法; 超低温保存; 茎尖; 病毒
收稿:2013-10-23 修定:2013-12-04
资助:四川农业大学“双支计划”项目(03570109)和四川农业大学大学生创新性实验计划(6309215)
Corresponding author: LUO Ya; E-mail: 173698873@qq.com; Tel: 13111822452
Abstract:
Cryotherapy is a novel application of plant cryopreservation in virus elimination. Due to high virusfree percentage and easy operation, it is considered to be the most effective detoxification method, and create a new path for the production of virus-free plantlets. In this paper, the principles, operating procedures, key technology and the latest research progress in horticultural plants of cryotherapy were summarized; the future development of cryotherapy was discussed as well.Key words: cryotherapy; cryopreservation; shoot tips; virus
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